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KMID : 0364320210460010031
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Journal of East-West Medicine
2021 Volume.46 No. 1 p.31 ~ p.38
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Anti-obesity Effect of Achyranthis Radix in 3T3-L1 Adipocytes and High Fat Diet-induced Obese C57BL/6 Mice
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Choi Jeong-Won
Roh Seong-Soo
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Abstract
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Objectives : The objective of this study was to confirm the anti-obesity effect of the Achyranthis Radix 30% EtOH extract (AE).
Methods : The AE treated with 100, 250, and 500 mg/mL on 3T3-L1 cells and then the cell viability was examined through MTT assay. In 3T3-L1 cells, Oil Red O staining was examined to detect triglyceride (TG) accumulation. Oil Red O staining for the examination of lipid accumulation was performed at concentrations 100, 250, and 500 mg/mL. Male C57BL/6 mice were fed on a high-fat diet (45% kcal fat; HFD) to induce obesity. Simultaneously, mice orally received 200 or 400 mg/kg AE for 6 weeks. Also, we measured the body weight gain and liver weight. Moreover, biochemical analyses including AST, ALT, TG, and TC levels were measured in serum.
Results : As a result of Oil Red O staining, both cell differentiation and TG accumulation were inhibited compared with Blank group. In the animal experiment, body weight gain in the AEH treated group was significantly suppressed. Moreover, liver weight gain was markedly suppressed in all drug groups. The increased liver function markers such as AST and ALT significantly decreased by AE treatment. Thus, serum TG and TC levels in AE groups showed a significant reduction compared with Control group.
Conclusion : Taken together, these results suggest that AE treatment showed anti-obesity effects by the inhibition of fat accumulation and the improvement of blood lipids. Therefore, AE could be utilized as a potential therapeutic agent for the prevention and treatment obesity.
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KEYWORD
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Achyranthis Radix, anti-obesity, 3T3-L1 cells, high fat diet, lipid accumulation
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